Duration: 3 days (18 credit hours)
Course Content:
Theoretical content:
- Basics of Reproductive Biology.
- Female Reproductive System.
- Oogenesis.
- ART– Importance, History, Applications and Methods.
- Oocytes Maturation.
- Hormonal Regulation of Male Reproduction.
- Spermatogenesis.
- Sperm Maturation and Capacitation.
- Mechanism of Fertilization.
- Embryo Development stages.
- Culture System and Media Composition.
- IVF Laboratory and Quality Management.
Practical part: - Recovery of oocytes; - Grading and selecting of oocytes; - Culturing oocytes for maturation. - Maturation Assessment; - Equilibration of Oocytes for IVF; - Sperm Preparation for IVF; - Perform IVF Step. - Embryos Quality Assessment; - Embryos Handling and Washing; - Culturing of Embryos in Specific Culture Medium.
Fees: 1200 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 2 days (12 credit hours)
Course Content:
Theoretical content:
- What stem cell is?
- Different types of stem cell.
- Hematopoietic Stem Cell.
- Mesenchymal stem cell.
- Applications of stem cell
Practical part:
- Dissecting out femur and tibia of mouse.
- Aspiration and culturing of bone marrow aspirates.
- Isolation and Culture of Hematopoietic Stem Cell.
- Stem Cell Counting.
- Sub-culturing of stem cell.
Fees: 1200 L.E. (Certificate, CofFees and lunch breaks are included) Maximum number of attendees 20 trainees
Duration: 2 days (12 credit hours)
Course Content:
Theoretical content:
- Tissue culture techniques.
- Basics of tissue culture.
- Types of Tissue culture.
- Different types of tissue culture media.
- Growth regulators.
- Tissue Culture Troubleshooting.
- Materials used in sterilization.
- Explant culture.
Practical part:
- Preparation and Sterilization of culture Media.
- Primary culture of plants embryos.
- Explant subculture.
Fees: 1000 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 2 days (12 credit hours)
Course Content:
Theoretical content:
• Intro to cancer biology
• Molecular mechanism of cancer
• What is RNA?
• Why we are interested in RNA?
• How to isolate RNA?
• How to judge RNA quality?
• Real-Time PCR
• Gene Expression data analysis
Practical part:
• RNA Isolation
• Quality Assessment of RNA
• cDNA Preparation
• cDNA Electrophoresis
• qPCR Preparation
• qPCR Results Monitoring
Fees: 1300 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 3 days (18 credit hours)
Course Content:
Theoretical content:
• LMO development
• The relevance of LMO detection in accordance to Cartagena protocol and national level
• Principals of quality control for LMO analysis
• Experimental design for LMO analysis
• Report writing
• Validation of LMO testing method
• Biosafety laws
• Biosafety clearing house mechanism
Practical part:
• DNA isolation( different validated methods )
• agarose gel preparation, gel loading
• pcr preparation
• gel interpretation
• nested primer
• real time pcr application in LMO detection
• strip analysis
Fees: 1200 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 3 days (18 credit hours)
Course Content:
• Databases and literature search
• DNA sequence retrieval and comparison
• Primer design
• Multiple sequence alignment and phylogenetic tree
Fees: 1200 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 4 days (24 credit hours)
Course Content:
Theoretical content:
• principal of gene expression
• application of PCR techniques
• Application of real-time PCR
• Real-time PCR data analysis
Practical part:
• RNA isolation
• cDNA synthesis
• PCR technique
• Performing real-time PCR analysis
Fees: 1500 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 4 days (24 credit hours)
Course Content:
• DNA extraction from bacteria
• Bacterial PCR based DNA fingerprints
• Bacterial identification based-on 16S rRNA gene sequencing.
Fees: 1200 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 3 days (18 credit hours)
Course Content:
• How to perform a differential expression analysis at the gene-level
• The steps and statistical approaches used in assessing the quality of your abundance estimates (count data)
• How to visualize expression patterns for differentially expressed genes
• How to perform functional analysis on gene lists.
Fees: 1500 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees
Duration: 3 days (18 credit hours)
Fees: 1300 L.E. (Certificate, CofFees and lunch breaks are included)
Maximum number of attendees 20 trainees