Genetic Characterization for Mycobacterium tuberculosis by Using DNA Barcode Genes of Chaperonin 60

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Accession No.: LC130572
Submitted (05-MAR-2016) to the DDBJ/EMBL/GenBank databases. Mycobacterium tuberculosis is an obligate pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of tuberculosis.The genome contains 250 genes involved in fatty acid metabolism, with 39 of these involved in the polyketide metabolism generating the waxy coat.

Such large numbers of conserved genes show the evolutionary importance of the waxy coat to pathogen survival. Chaperonin sequences are useful for phylogenetic studies and have been widely exploited in studies of prokaryotic and eukaryotic evolution. Group I chaperonin sequences have also been employed as targets for detection and identification of organisms since a 549-567 bp segment of the cpn60 coding region, the "universal target", can be amplified with universal PCR primers.

DNA Barcodes to Identify and Classify Bacterial strain (Main Steps to isolate Cpn60 gene):

1.Primers Design Specific to Barcoding Gene.
2.Collected from :sputum culture from Abbasseya Chest Hospital
3.DNA Isolation.
4.PCR for Barcoding Gene.
5.Gel Purification Method.
6.DNA Sequencing for Barcoding Gene.
7.Bioinformatics Analysis.
8.Genbank Submission.
9.Dendrogram.